Some considerations in the preparation of raioiodoinsulin for radioimmunoassay and receptor assay.

@article{Schneider1976SomeCI,
  title={Some considerations in the preparation of raioiodoinsulin for radioimmunoassay and receptor assay.},
  author={Barbara Taback Schneider and Eugene Straus and Rosalyn Sussman Yalow},
  journal={Diabetes},
  year={1976},
  volume={25 4},
  pages={260-7}
}
125I-insulins, prepared by iodination with chloramine T in marked excess or by stepwise, stoichiometric addition of the oxidizing agent, were compared with respect both to their molecular distribution of iodine and to their suitability for use in a cultured lymphocyte receptor assay. Iodination of insulin in aqueous solution results in the same distribution of iodine atoms, independent of experimental method and dependent only on the average iodine number. This distribution can be calculated on… CONTINUE READING

Citations

Publications citing this paper.

Similar Papers

Loading similar papers…