Some characteristics of the dihydrofolate synthetase from Serratia indica.

  title={Some characteristics of the dihydrofolate synthetase from Serratia indica.},
  author={Makoto Ikeda and Kazuo Iwai},
  journal={Journal of nutritional science and vitaminology},
  volume={22 5},
  • M. Ikeda, K. Iwai
  • Published 1976
  • Chemistry, Biology
  • Journal of nutritional science and vitaminology
Dihydrofolate synthetase (EC from Serratia indica IFO 3759 require a divalent cation and a univalent cation for its activity. The divalent cation requirement was satisified by magnesium ion, manganese ion or ferrous ion. High activity was obtained with 5 mM of magnesium ion. The effect of manganese ion was weak. The univalent cation requirement was satisfied by potassium ion, ammonium ion or rubidium ion, and high activity was obtained with 100 mM of each univalent cation. Increase in… 
2 Citations
Characterization and inhibition of dihydrofolate synthetase from Neisseria gonorrhoeae
Dihydrofolate synthetase (EC 6.3.12) from N. gonorrhoeae was isolated and enzyme characteristics were determined, and patterns of product inhibition by dihydrofolates were found to be non-competitive with respect to dihydropteroate, and competitive withrespect to L-glutamate.
Nutritional requirements for folate compounds and some enzyme activities involved in the folate biosynthesis.
The nutritional requirements for folate compounds shown in vivo is in good agreement with the results obtained with dihydropteroate and dihydrofolate synthetase activities in vitro, and that the pathway through dihydopteroic acid as an intermediate is the main route in folate biosynthesis in nature.


Experimental Chemical Handbook
  • 1966
Editor), Methods in Enzymology
  • Part B, p
  • 1971