Some causes of inter-laboratory variation in the results of comet assay.

@article{Sirota2014SomeCO,
  title={Some causes of inter-laboratory variation in the results of comet assay.},
  author={Nikolai P Sirota and Aliy K. Zhanataev and E. A. Kuznetsova and Eugenii P Khizhnyak and Elena A Anisina and Andrei D Durnev},
  journal={Mutation research. Genetic toxicology and environmental mutagenesis},
  year={2014},
  volume={770},
  pages={16-22}
}
We performed an inter-laboratory study to determine the variation of comet assay results and to identify its possible reasons. An exchange of slides between Labs in different stages of the comet assay protocol was performed. Because identical slides, durations of alkali treatment and electrophoresis, and similar electric field strengths (2.0 V/cm and 2.14 V/cm) were used, we concluded that the observed inter-laboratory difference in the results is directly associated with the electrophoresis… CONTINUE READING