Somatostatin inhibition of phosphoinositides turnover in isolated rat acinar pancreatic cells: interaction with bombesin

@article{Linard1992SomatostatinIO,
  title={Somatostatin inhibition of phosphoinositides turnover in isolated rat acinar pancreatic cells: interaction with bombesin},
  author={C. Linard and F. Reyl-Desmars and M. Lewin},
  journal={Regulatory Peptides},
  year={1992},
  volume={41},
  pages={219-226}
}
The effects of somatostatin-14 and bombesin on [3H]inositol phosphate accumulation were studied in 24 h myo-[3H]inositol-prelabeled cultured rat acinar cells. Bombesin, 10 nM, stimulated basal formation of phosphatidyl monophosphate (InsP1), phosphatidyl 4,5-biphosphate (InsP2) and inositol 1,4,5-triphosphate (InsP3) by 128 +/- 5.2%, 147 +/- 10% and 155 +/- 5%, respectively. At 5 s, the ED50 value for InsP3 stimulation was 0.70 +/- 0.2 nM. This stimulation was partly blocked (64 +/- 0.04… Expand
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References

SHOWING 1-10 OF 25 REFERENCES
Intracellular mediators of bombesin action on rat pancreatic acinar cells.
TLDR
The results suggest that bombesin receptors initiate similar intracellular messengers as does CCK, however, CCK induces a larger increase of diacylglycerol and probably an as yet unidentified messenger responsible for its inhibitory effects. Expand
Intracellular receptor for somatostatin in gastric mucosal cells: decomposition and reconstitution of somatostatin-stimulated phosphoprotein phosphatases.
  • F. Reyl, M. Lewin
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1982
TLDR
The results strongly argue for the presence of an intracellular somatostatin receptor in gastric mucosal cells and characterize this receptor as a PPPase regulatory subunit. Expand
A somatostatin receptor negatively coupled to adenylate cyclase in the human gastric cell line HGT-1
TLDR
Somatostatin binding to the high affinity sites was partially inhibited by the non-hydrolysable guanyl nucleotide analog Gpp(NH)p and by pretreating the cells with islet activating protein (IAP). Expand
Bombesin stimulates inositol polyphosphate production in GH4C1 pituitary tumor cells: comparison with TRH.
TLDR
Maximal stimulation of inositol-1,4,5-trisphosphate formation by TRH was not further increased by addition of a maximally effective dose of bombesin, suggesting that the two hormones act through stimulation of a common pool of phospholipase C, and this enzyme pool can be fully stimulated byTRH, alone. Expand
Role of Ca2+ influx in bombesin-induced mitogenesis in Swiss 3T3 fibroblasts.
TLDR
The results suggest that the target of Ca2+ influx in bombesin-induced mitogenic signaling pathway is not located along the phospholipase C-protein kinase C signal transduction system including cellular events in early G1 phase that exist downstream to protein kinases C action. Expand
Coupling of guanine nucleotide inhibitory protein to somatostatin receptors on pancreatic acinar membranes.
TLDR
The present results suggest that somatostatin probably functions in the pancreas to regulate adenylate cyclase enzyme system via Ni, the extent of modification of Ni is correlated with the ability of somatstatin to inhibit cAMP accumulation in acini, and guanine nucleotides also inhibit som atostatin binding to its receptor. Expand
Interaction of [125I]-Tyr4-bombesin with specific receptors on normal human pancreatic membranes
TLDR
The binding of bombesin to its receptors on normal human pancreatic membranes was investigated using high specific activity, radioiodinated bombsin ([125I]-Tyr4-bombesin, prepared by an oxidative method with chloramine-T and the binding characteristics obtained are comparable with those found in other species. Expand
Thyrotropin-releasing hormone rapidly activates the phosphodiester hydrolysis of polyphosphoinositides in GH3 pituitary cells. Evidence for the role of a polyphosphoinositide-specific phospholipase C in hormone action.
  • T. Martin
  • Biology, Medicine
  • The Journal of biological chemistry
  • 1983
TLDR
Results indicate that TRH rapidly causes activation of a polyphosphoinositide-hydrolyzing phospholipase C-type enzyme and suggests a proximal role for polyph phosphate breakdown in the sequence of events by which TRH alters pituitary cell function. Expand
Evidence for an intracellular somatostatin receptor in pancreas: a comparative study with reference to gastric mucosa.
TLDR
It is suggested that in pancreas as in gastric mucosa, somatostatin's inhibitory effect on secretory functions could involve protein dephosphorylation mediated by an intracellular receptor. Expand
Somatostatin inhibits cAMP-dependent and cAMP-independent calcium influx in the clonal pituitary tumor cell line AtT-20 through the same receptor population.
TLDR
The ability of SRIF to block CRF (cyclic AMP-dependent) and K+-stimulated calcium influx into AtT-20 cells cannot be separated. Expand
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