Somatic exocytosis of serotonin mediated by L-type calcium channels in cultured leech neurones.

@article{Trueta2003SomaticEO,
  title={Somatic exocytosis of serotonin mediated by L-type calcium channels in cultured leech neurones.},
  author={Citlali Trueta and Bruno M{\'e}ndez and Francisco F. De-Miguel},
  journal={The Journal of physiology},
  year={2003},
  volume={547 Pt 2},
  pages={
          405-16
        }
}
We studied somatic exocytosis of serotonin and its mediation by L-type calcium (Ca2+) channels in cultured Retzius neurones of the leech. Exocytosis was induced by trains of impulses at different frequencies or by depolarisation with 40 mM potassium (K+), and was quantified by use of the fluorescent dye FM 1-43. Stimulation increased the membrane fluorescence and produced a pattern of FM 1-43 fluorescent spots of 1.28 +/- 0.01 microm in diameter, provided that Ca2+ was present in the bathing… CONTINUE READING
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