Somatic embryogenesis in avocado (Persea americana Mill.) in vitro

  title={Somatic embryogenesis in avocado (Persea americana Mill.) in vitro},
  author={Fernando Pliego-Alfaro and Toshio Murashige},
  journal={Plant Cell, Tissue and Organ Culture},
A tissue culture procedure was developed for the regeneration of somatic embryos from callus cultures of the avocado,Persea americana. Immature zygotic embryos, 0.6–0.8 mm long, were used as original explants. Addition of 0.1 mg/l picloram (4-amino-3,5,6-trichloropicolinic acid) to culture medium appeared critical for callus initiation. Development of somatic embryos was accomplished in picloram concentrations of 0.01 to 0.1 ml/l. A few well developed embryos produced green shoots. Attempts to… CONTINUE READING
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