Somatic embryogenesis from Sorghum bicolor leaves

@article{Wernicke1980SomaticEF,
  title={Somatic embryogenesis from Sorghum bicolor leaves},
  author={Wolfgang Wernicke and Richard I. S. Brettell},
  journal={Nature},
  year={1980},
  volume={287},
  pages={138-139}
}
Plant regeneration from totipotent cultured cells or protoplasts is a prerequisite for the often proposed genetic modification of plants through somatic cell genetics, and has been achieved in many species. The cereals (and the rest of the Gramineae) have, however, proved to be extremely unresponsive to in vitro culture techniques1. The most convenient source of plant protoplasts is the leaf tissue because it allows the isolation of a large, relatively uniform cell population without the… CONTINUE READING
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