Somatic embryogenesis and plant regeneration of Vitis vinifera cultivars 'Macabeo' and 'Tempranillo'

Abstract

Different experimental conditions have been compared to achieve a high efficiency in embryogenic calli initiation from 'Macabeo' and 'Tempranillo' anthers. Specifically, two stages of anther development were tested (corresponding to tetrad cells or uninucleate pollen), and direct culture of anthers was compared to culture after a cold treatment of… (More)

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