Somatic embryogenesis and organogenesis of Agave victoriae-reginae: Considerations for its conservation

@article{MartnezPalacios2004SomaticEA,
  title={Somatic embryogenesis and organogenesis of Agave victoriae-reginae: Considerations for its conservation},
  author={Alejandro Mart{\'i}nez-Palacios and Mar{\'i}a del Pilar Ortega-Larrocea and V{\'i}ctor M. Ch{\'a}vez and Robert Bye},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={74},
  pages={135-142}
}
Agave victoriae-reginae somatic embryos were produced through a callus phase from seedling stem segments cultured on MS medium. The optimal treatment was MS medium with 2.26 μM 2,4-D. Multiple shoot regeneration was induced from axillary buds from stem segments cultured on MS medium with 2.2–4.4 μM BA. Effect of MS and modified MS medium with 50% macronutrient concentration, both containing 2.2 μM BA and sucrose at the following concentrations, 20, 30, 45 and 60 g l−1, resulted in inconsistent… CONTINUE READING
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