Somatic embryo cycling: Evaluation of a novel transformation and assay system for seed-specific gene expression in soybean

@article{Liu2006SomaticEC,
  title={Somatic embryo cycling: Evaluation of a novel transformation and assay system for seed-specific gene expression in soybean},
  author={Wennuan Liu and Rebecca S. Torisky and Kay P McAllister and Sergei A. Avdiushko and David Hildebrand and Glenn B. Collins},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2006},
  volume={47},
  pages={33-42}
}
Somatic embryo cycling, a modification of soybean somatic embryogenic suspension culture, was developed as an efficient and rapid method of producing tissue suitable for stable transformation of soybean germplasm by biolistic particle bombardment. Instead of using immature seed explants, cotyledon-staged somatic embryo hypocotyls were placed on auxin-containing medium, where they initiated new somatic embryos primarily from single epidermal cells. By bombarding hypocotyls prior to initiation of… CONTINUE READING
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