Somatic cell nuclear transfer in zebrafish

@article{Siripattarapravat2009SomaticCN,
  title={Somatic cell nuclear transfer in zebrafish},
  author={Kannika Siripattarapravat and Boonya Pinmee and Patrick J. Venta and Chia-Cheng Chang and Jose Bernardo Cibelli},
  journal={Nature Methods},
  year={2009},
  volume={6},
  pages={733-735}
}
We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones. 

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