Somatic Seeds: Encapsulation of Asexual Plant Embryos

  title={Somatic Seeds: Encapsulation of Asexual Plant Embryos},
  author={Keith Redenbaugh and Brian D. Paasch and James W. Nichol and Mary E. Kossler and Peter R. Viss and Keith A. Walker},
Somatic embryos (SE) of alfalfa, celery, and cauliflower were encapsulated as single–embryo beads approximately four millimeters in diameter to produce individual somatic (artificial) seeds. The SE were mixed in sodium alginate and dropped into a solution of calcium chloride to form calcium alginate beads via an ion exchange reaction. For our two best somatic embryogenesis systems, alfalfa and celery, in vitro germination frequencies with production of seedling–quality plants were routinely 30… CONTINUE READING
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