Solvent Extraction and Chromatographic Determination of Polyphenols in Olive Pomace

Abstract

Olive oil mill waste obtained after two-phase olive oil extraction process was subjected to conventional liquid solvent extraction under different pH/temperature/duration conditions using different types of food-grade solvents. The independent variables were: solvent type (ethanol percentage), extraction temperature (20-90 °C), extraction time (30 min-24 h) and pH (210.3) of extraction solvent while the response variables were total phenolic content and antioxidant activity of obtained extracts. The optimum solvent extraction conditions for phenols were 120 min at 70 °C using 60% ethanol as extraction solvent, at solvent to sample ratio 5:1 (v/w). For quantification of major bioactive olive polyphenols hydroxytyrosol, tyrosol and oleuropein in obtained extracts, fast and simple RP-HPLC-DAD method was developed and validated. Oleuropein was presented in highest amounts with average value of 115.14±0.19 mg/kg of fresh olive pomace.

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Cite this paper

@inproceedings{epo2017SolventEA, title={Solvent Extraction and Chromatographic Determination of Polyphenols in Olive Pomace}, author={Dubravka Vitali {\vC}epo and Petra Albahari and Marijana Zovko Kon{\vc}i{\'c} and Kristina Radi{\'c} and Sanja Jurmanovi{\'c} and Mario Jug}, year={2017} }