Solutions to problems in enumerating sediment bacteria by direct counts.

  title={Solutions to problems in enumerating sediment bacteria by direct counts.},
  author={M Schallenberg and Jacob Kalff and Joseph B. Rasmussen},
  journal={Applied and environmental microbiology},
  volume={55 5},
We examined the effect of different sediment types on the staining effectiveness of the fluorochrome DAPI (4'-6-diamidino-2-phenylindole dihydrochloride) over a wide range of concentrations and on the masking effect of sediment particles on DAPI-stained bacteria. Sediment type greatly affects the staining efficiency of DAPI, and most published studies seem to have underestimated bacterial abundances by using suboptimal concentrations of the fluorochrome. A DAPI concentration of 5 mug ml is… CONTINUE READING

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