Solution structure of the antiapoptotic protein bcl-2.

  title={Solution structure of the antiapoptotic protein bcl-2.},
  author={Andrew M. Petros and Ales Medek and David G. Nettesheim and Daniel H Kim and Ho Sup Yoon and Kathy Swift and Edmund D. Matayoshi and Tilman Oltersdorf and Stephen W. Fesik},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={98 6},
The structures of two isoforms of Bcl-2 that differ by two amino acids have been determined by NMR spectroscopy. Because wild-type Bcl-2 behaved poorly in solution, the structures were determined by using Bcl-2/Bcl-x(L) chimeras in which part of the putative unstructured loop of Bcl-2 was replaced with a shortened loop from Bcl-x(L). These chimeric proteins have a low pI compared with the wild-type protein and are soluble. The structures of the two Bcl-2 isoforms consist of 6 alpha-helices with… CONTINUE READING
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