Solution structure of calcium-free calmodulin

@article{Kuboniwa1995SolutionSO,
  title={Solution structure of calcium-free calmodulin},
  author={Hitoshi Kuboniwa and Nico Tjandra and Stephan Grzesiek and Hao Ren and Claude B. Klee and Adriaan Bax},
  journal={Nature Structural Biology},
  year={1995},
  volume={2},
  pages={768-776}
}
The three-dimensional structure of calmodulin in the absence of Ca2+ has been determined by three- and four-dimensional heteronuclear NMR experiments, including ROE, isotope-filtering combined with reverse labelling, and measurement of more than 700 three-bond J-couplings. In analogy with the Ca2+-ligated state of this protein, it consists of two small globular domains separated by a flexible linker, with no stable, direct contacts between the two domains. In the absence of Ca2+, the four… Expand
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