Solution structure of ThiS and implications for the evolutionary roots of ubiquitin

@article{Wang2001SolutionSO,
  title={Solution structure of ThiS and implications for the evolutionary roots of ubiquitin},
  author={Chunyu Wang and Jun Xi and Tadhg P. Begley and Linda K. Nicholson},
  journal={Nature Structural Biology},
  year={2001},
  volume={8},
  pages={47-51}
}
ThiS is a sulfur carrier protein that plays a central role in thiamin biosynthesis in Escherichia coli. Here we report the solution NMR structure of ThiS, the first for this class of sulfur carrier proteins. Although ThiS shares only 14% sequence identity with ubiquitin, it possesses the ubiquitin fold. This structural homology, combined with established functional similarities involving sulfur chemistry, demonstrates that the eukaryotic ubiquitin and the prokaryotic ThiS evolved from a common… 

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