Solution structure, divalent metal and DNA binding of the endonuclease domain from the replication initiation protein from porcine circovirus 2.

@article{VegaRocha2007SolutionSD,
  title={Solution structure, divalent metal and DNA binding of the endonuclease domain from the replication initiation protein from porcine circovirus 2.},
  author={Susana Vega-Rocha and In-Ja L. Byeon and Bruno Gronenborn and Angela M. Gronenborn and Ram{\'o}n Campos-Olivas},
  journal={Journal of molecular biology},
  year={2007},
  volume={367 2},
  pages={473-87}
}
Circoviruses are the smallest circular single-stranded DNA viruses able to replicate in mammalian cells. Essential to their replication is the replication initiator, or Rep protein that initiates the rolling circle replication (RCR) of the viral genome. Here we report the NMR solution three-dimensional structure of the endonuclease domain from the Rep protein of porcine circovirus type 2 (PCV2), the causative agent of postweaning multisystemic wasting syndrome in swine. The domain comprises… CONTINUE READING