Solution scattering structural analysis of the 70 S Escherichia coli ribosome by contrast variation. II. A model of the ribosome and its RNA at 3.5 nm resolution.

@article{Svergun1997SolutionSS,
  title={Solution scattering structural analysis of the 70 S Escherichia coli ribosome by contrast variation. II. A model of the ribosome and its RNA at 3.5 nm resolution.},
  author={Dmitri I. Svergun and Nils Burkhardt and Jan Skov Pedersen and Michel H. J. Koch and Victor V. Volkov and Mikhail B. Kozin and W Meerwink and Heinrich B. Stuhrmann and G Diedrich and Knud H. Nierhaus},
  journal={Journal of molecular biology},
  year={1997},
  volume={271 4},
  pages={602-18}
}
Selectively deuterated 70 S E. coli ribosomes and isolated 30 S and 50 S subunits were analyzed by X-ray and neutron solution scattering. The resulting contrast variation data set (42 curves in total) was proven to be consistent in describing the ribosome as a four-phase system composed of the protein and rRNA moieties of both subunits. This data set thus provides ten times more information than a single scattering curve. A solid body four-phase model of the 70 S ribosome at low resolution was… CONTINUE READING

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