Solution properties and computational analysis of an oligodeoxynucleotide containing N-(deoxyguanosin-8-yl)-1-aminopyrene.

@article{Nolan1996SolutionPA,
  title={Solution properties and computational analysis of an oligodeoxynucleotide containing N-(deoxyguanosin-8-yl)-1-aminopyrene.},
  author={Scott J. Nolan and Rejeev R. Vyas and Brian E. Hingerty and S. Ellis and Suse Broyde and Robert Shapiro and Ashis K Basu},
  journal={Carcinogenesis},
  year={1996},
  volume={17 1},
  pages={
          133-44
        }
}
An oligodeoxyribonucleotide 5'-d(CTCATGAPATTCC), in which G(AP) denotes N-(guanin-8-yl)-1-aminopyrene, the C8-guanine adduct of reductively activated 1-nitropyrene, was synthesized and characterized by polyacrylamide gel electrophoresis, absorption and fluorescence spectroscopy, circular dichroism, and thermal melting studies. Polyacrylamide gel electrophoresis showed slower mobility of the adducted oligonucleotide in single-stranded form compared to its unmodified counterpart, as expected. In… Expand
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Two-dimensional proton NMR and energy minimization computations have been employed to characterize the conformations of the N-(deoxyguanosin-8-yl)aminofluorene adduct positioned opposite deoxyguanoine in one DNA undecamer duplex in aqueous solution, finding a conformation similar to that found previously when the (AF)G was positionedosite deoxyadenosine. Expand
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1-Nitropyrene, the most abundant nitro-polycyclic aromatic hydrocarbon in the environment, is a known mammalian and bacterial mutagen and a tumorigen in animals. Early studies on DNA adductExpand
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