Solution-phase DNA mutation scanning and SNP genotyping by nanoliter melting analysis.

@article{Sundberg2007SolutionphaseDM,
  title={Solution-phase DNA mutation scanning and SNP genotyping by nanoliter melting analysis.},
  author={Scott O. Sundberg and Carl T. Wittwer and Jenny Greer and Robert J. Pryor and Oluwole Elenitoba-Johnson and Bruce K. Gale},
  journal={Biomedical microdevices},
  year={2007},
  volume={9 2},
  pages={159-66}
}
Solution-phase, DNA melting analysis for heterozygote scanning and single nucleotide polymorphism (SNP) genotyping was performed in 10 nl volumes on a custom microchip. Human genomic DNA was PCR amplified in the presence of the saturating fluorescent dye, LCGreen Plus, and placed within microfluidic channels that were created between two glass slides. The microchip was heated at 0.1 degrees C/s with a Peltier device and viewed with an inverted fluorescence microscope modified for… CONTINUE READING
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