Solution conformation of the major adduct between the carcinogen (+)-anti-benzo[a]pyrene diol epoxide and DNA.

  title={Solution conformation of the major adduct between the carcinogen (+)-anti-benzo[a]pyrene diol epoxide and DNA.},
  author={Monique Cosman and Carlos R. de los Santos and Radovan Fiala and Brian E. Hingerty and S. B. Singh and V{\'i}ctor Ib{\'a}{\~n}ez and Leonid A. Margulis and David Live and Nicholas E. Geacintov and Suse Broyde},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  pages={1914 - 1918}
We have synthesized, separated, and purified approximately 10 mg of a deoxyundecanucleotide duplex containing a single centrally positioned covalent adduct between (+)-anti-benzo[a]pyrene (BP) diol epoxide and the exocyclic amino group of guanosine. Excellent proton NMR spectra are observed for the (+)-trans-anti-BP diol epoxide-N2-dG adduct positioned opposite dC and flanked by G.C pairs in the d[C1-C2-A3-T4-C5-(BP)G6-C7-T8-A9-C10-C11].d[12- G13-T14-A15-G16-C17-G18-A19-T20-G 21-G22] duplex… 

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