Solution NMR spin relaxation methods for characterizing chemical exchange in high-molecular-weight systems.

@article{Palmer2005SolutionNS,
  title={Solution NMR spin relaxation methods for characterizing chemical exchange in high-molecular-weight systems.},
  author={Arthur G. Palmer and Michael J Grey and Chunyu Wang},
  journal={Methods in enzymology},
  year={2005},
  volume={394},
  pages={430-65}
}
Transverse relaxation optimized NMR spectroscopy (TROSY) techniques for (1)H-(15)N backbone amide moieties and for (13)CH(3) methyl groups have permitted the development of Hahn spin echo and Carr-Purcell-Meiboom-Gill (CPMG) experiments for characterizing chemical exchange kinetic phenomena on microsecond-millisecond time scales in proteins with molecular masses >50 kDa. This chapter surveys the theoretical bases for TROSY in spin systems subject to chemical exchange linebroadening, the… CONTINUE READING