Solution NMR of proteins within polyacrylamide gels: diffusional properties and residual alignment by mechanical stress or embedding of oriented purple membranes.

@article{Sass2000SolutionNO,
  title={Solution NMR of proteins within polyacrylamide gels: diffusional properties and residual alignment by mechanical stress or embedding of oriented purple membranes.},
  author={H J Sass and Giovanna Musco and Stephen J. Stahl and Paul T Wingfield and Stephan Grzesiek},
  journal={Journal of biomolecular NMR},
  year={2000},
  volume={18 4},
  pages={303-9}
}
The diffusive properties of biomacromolecules within the aqueous phase of polyacrylamide gels are described. High quality NMR spectra can be obtained under such conditions. As compared to water, a fivefold reduction in the translational diffusion constant, but only a 1.6-fold decrease (1.4-fold increase) in amide-15N T2 (T1) are observed for human ubiquitin within a 10% acrylamide gel. Weak alignment of the solute macromolecules can be achieved within such gels by vertical or radial compression… CONTINUE READING

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