Solute accumulation differs in the vacuoles and apoplast of ripening grape berries

@article{Keller2013SoluteAD,
  title={Solute accumulation differs in the vacuoles and apoplast of ripening grape berries},
  author={Markus Keller and Pradeep Shrestha},
  journal={Planta},
  year={2013},
  volume={239},
  pages={633-642}
}
Phloem unloading is thought to switch from a symplastic route to an apoplastic route at the beginning of ripening in grape berries and some other fleshy fruits. [] Key Method We modified a method developed for tomato fruit to extract apoplastic sap from grape berries and measured the changes in apoplastic and vacuolar pH, soluble sugars, organic acids, and potassium in ripening berries of Vitis vinifera ‘Merlot’ and V. labruscana ‘Concord’. Solute accumulation varied by genotype, compartment, and chemical…

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References

SHOWING 1-10 OF 56 REFERENCES
Fruit ripening in Vitis vinifera: apoplastic solute accumulation accounts for pre-veraison turgor loss in berries
TLDR
It is concluded that high concentrations of solutes accumulated in the mesocarp apoplast prior to veraison, and that P loss was a direct result of decreased ΨsA, which decreased before veraisons, suggesting that apoplastic solutes play an important role in the events of cellular metabolism that lead to the onset of ripening.
A Shift of Phloem Unloading from Symplasmic to Apoplasmic Pathway Is Involved in Developmental Onset of Ripening in Grape Berry1
TLDR
The occurrence of an adaptive shift of phloem unloading pathway to developmental transition from growing phase to ripening in grape berry is demonstrated clearly.
Changes in acidity and in proton transport at the tonoplast of grape berries during development
TLDR
The data indicate that tonoplast leakage dramatically increased during ripening, which is probably the prime cause of malate decompartmentation, amplified by the incapacity of oxidative phosphorylation to face increased energy demand.
Apoplastic pH and inorganic ion levels in tomato fruit: A potential means for regulation of cell wall metabolism during ripening
Apoplastic pH and ionic conditions exert strong influence on cell wall metabolism of many plant tissues; however, the nature of the apoplastic environment of ripening fruit has been the subject of
Phloem unloading follows an extensive apoplasmic pathway in cucumber (Cucumis sativus L.) fruit from anthesis to marketable maturing stage.
TLDR
There is clear evidence for an apoplasmic phloem unloading pathway in cucumber fruit and a presumption that putative raffinose or stachyose transporters may be involved in soluble sugars unloading was discussed.
Evidence for Apoplasmic Phloem Unloading in Developing Apple Fruit1
TLDR
Clear evidence is provided for an apoplasmic phloem unloading pathway in apple fruit and information is given on the structural and molecular features involved in this process.
Grape Berry Vacuole: A Complex and Heterogeneous Membrane System Specialized in the Accumulation of Solutes
TLDR
The present review focuses on the storage function of vacuoles and on their structure and diversity in relation to development and ripening of the grape berry.
The cellular pathway of postphloem sugar transport in developing tomato fruit
TLDR
The cellular pathway of postphloem sugar transport was elucidated in the outer pericarp of tomato fruit at 13–14 and 23–25 days after anthesis (DAA) to indicate that an energy-coupled plasma-membrane hexose carrier is expressed specifically in storage parenchyma cells at the latter stage of fruit development.
The Composition of Apoplast Fluid Recovered From Intact Developing Tomato Fruit
A pressure dehydration technique for recovering sap from the apoplast of the pericarp tissue of developing tomato fruit has recently been developed. Samples of this sap from two cultivars have now
Levels of apoplastic solutes in developing strawberry fruit
TLDR
Three methods were used to collect apoplastic solution from developing strawberry fruit for determiningApoplastic solute potential (ψ as ) and sucrose and glucose concentrations and liquid on filter paper discs inserted into the hollow cavity naturally formed in ripening fruit suggested leakage of anthocyanins into the apoplast.
...
1
2
3
4
5
...