Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice.

@article{Vank2008SolubleRC,
  title={Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice.},
  author={Ondřej Vaněk and Monika N{\'a}lezkov{\'a} and Daniel Kavan and Ivana Borovickov{\'a} and Petr Pompach and Petr Nov{\'a}k and Vinay Kumar and Luca Vannucci and Jiř{\'i} Hude{\vc}ek and Kateřina Hofbauerov{\'a} and Vladim{\'i}r Kopeck{\'y} and J{\'i}r{\'i} Brynda and Petr Kolenko and Jan Dohn{\'a}lek and Pavel Kadeř{\'a}vek and Josef Chmel{\'i}k and Luk{\'a}s Gorc{\'i}k and Luk{\'a}{\vs} Z{\'i}dek and Vladim{\'i}r Sklen{\'a}ř and Karel Bezou{\vs}ka},
  journal={The FEBS journal},
  year={2008},
  volume={275 22},
  pages={5589-606}
}
We investigated the soluble forms of the earliest activation antigen of human leukocyte CD69. This receptor is expressed at the cell surface as a type II homodimeric membrane protein. However, the elements necessary to prepare the soluble recombinant CD69 suitable for structural studies are a matter of controversy. We describe the physical, biochemical and in vivo characteristics of a highly stable soluble form of CD69 obtained by bacterial expression of an appropriate extracellular segment of… CONTINUE READING

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This receptor is expressed at the cell surface as a type II homodimeric membrane protein .
This receptor is expressed at the cell surface as a type II homodimeric membrane protein .
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