Soluble interleukin‐6 receptors released from T cell or granulocyte/macrophage cell lines and human peripheral blood mononuclear cells are generated through an alternative splicing mechanism

@article{Horiuchi1994SolubleIR,
  title={Soluble interleukin‐6 receptors released from T cell or granulocyte/macrophage cell lines and human peripheral blood mononuclear cells are generated through an alternative splicing mechanism},
  author={Sankichi Horiuchi and Yoshio Koyanagiu and Yongwei Zhouu and Hirokuni Miyamotou and Yuetsu Tanakau and Michinori Waki and Akiyoshi Matsumoto and Mikio Yamamotou and Naoki Yamamotof},
  journal={European Journal of Immunology},
  year={1994},
  volume={24}
}
To detect transcripts encoding the interleukin‐6 receptor (IL‐6R) molecule lacking the transmembrane (TM) domain, in various cell lines and peripheral blood mononuclear cells (PBMC), we used the polymerase chain reaction (PCR) with primer pairs that flank the TM domain and which were selected to generate a 398‐bp fragment. We detected 398‐bp and 304‐bp DNA molecules in the PCR products of the U1, J22HL60, MT‐2, MT‐4, U937 and HL60 cell lines and of PBMC isolated from several individuals. The… 
View on Wiley
ncbi.nlm.nih.gov
211 Citations
Highly Influential Citations
5
Background Citations
64
Methods Citations
6
Results Citations
1

211 Citations

High‐level production of alternatively spliced soluble interleukin‐6 receptor in serum of patients with adult T‐cell leukaemia/HTLV‐I‐associated myelopathy
TLDR
It is confirmed that alternative splicing of IL‐6R mRNA is of consequence in ATL, HAM and in some autoimmune diseases, and the HTLV‐I‐infected T’cells appeared to play an important role in AS‐sIL‐ 6R production.
Major role of the soluble interleukin‐6/interleukin‐6 receptor complex for the proliferation of interleukin‐6‐dependent human myeloma cell lines
TLDR
The results show that the levels of circulating sIL‐ 6R (and thus those of IL‐6/sIL‐6R complex) are worth looking at in pathologies involving IL‐8 hyperactivity, and the major role of the sIL-6R for sustaining the proliferation of these cell lines.
Multilevel Regulation of IL‐6R by IL‐6–sIL‐6R Fusion Protein According to the Primitiveness of Peripheral Blood‐Derived CD133+ Cells
TLDR
It is assumed that sIL‐6R produced by shedding should be involved in autocrine and paracrine loops in the HSC microenvironment and expression and production of IL‐ 6R are tightly regulated and stage specific.
Human T‐cell leukemia virus type‐I Tax induces expression of interleukin‐6 receptor (IL‐6R): Shedding of soluble IL‐6R and activation of STAT3 signaling
Human T‐cell leukemia virus type‐I (HTLV‐I) encodes for the viral protein Tax, which is known to significantly disrupt transcriptional control of cytokines, cytokine receptors and other…
Soluble interleukin-6 receptor enhanced by oncostatin M induces major changes in gene expression profile of human hepatoma cells.
Shedding of the soluble IL‐6 receptor is triggered by Ca2+ mobilization, while basal release is predominantly the product of differential mRNA splicing in THP‐1 cells
TLDR
The divergent control of these sIL‐6R isoforms indicates that they may independently influence the inflammatory response.
Acute myeloblastic leukaemia cells produce soluble interleukin 6 receptor by a mechanism of alternative splicing.
Soluble interleukin‐6 receptor strongly increases the production of acute‐phase protein by hepatoma cells but exerts minimal changes on human primary hepatocytes
TLDR
The results show that the expression of IL‐ 6R is low in the hepatoma cell PLC/PRF/5 when compared with primary hepatocytes and that this difference can, at least partly, explain their deficient responsiveness to IL‐6.
Colony-Stimulating Factor Receptors from Production of Soluble Granulocyte
TLDR
Two isoforms of sG-CSFR are physiologically secreted from relatively mature myeloid cells and might play an important role in myelopoiesis through their binding to serum G-CSF.
Production of soluble granulocyte colony-stimulating factor receptors from myelomonocytic cells.
TLDR
Two isoforms of sG-CSFR are physiologically secreted from relatively mature myeloid cells and might play an important role in myelopoiesis through their binding to serum G-CSF.
...
...

References

SHOWING 1-10 OF 15 REFERENCES
Soluble cytokine receptors are present in normal human urine
TLDR
This finding, together with the already known presence of urinary TNF binding proteins and a soluble IL-2-R both in plasma and in urine, indicates that release of soluble cytokine receptors into body fluids is a general phenomenon that occurs under normal physiological conditions.
Identification of a neutralization epitope on the envelope gp46 antigen of human T cell leukemia virus type I and induction of neutralizing antibody by peptide immunization.
TLDR
Results show that the HTLV-I neutralization epitope recognized by LAT-27 locates to the gp46 amino acids 191-196, and that immunization with a peptide containing the LAT- 27 epitope can elicit an HTLV -I neutralizing antibody response.
Human B-cell differentiation factor defined by an anti-peptide antibody and its possible role in autoantibody production.
  • T. HiranoT. Taga F. Sakiyama
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1987
TLDR
The results show that B SF-2 functions in vivo as well and suggest that the constitutive production of BSF-2 may be involved in autoantibody production, since patients with cardiac myxoma and uterine carcinoma showed autoant ibody production.
Analysis of rev gene function on human immunodeficiency virus type 1 replication in lymphoid cells by using a quantitative polymerase chain reaction method
TLDR
The conventional polymerase chain reaction method is modified for general use as an extremely sensitive procedure for quantitative analysis of RNA species and shows that the ultimate effect of Rev is to direct the appearance of unspliced or singly spliced RNAs in the cytoplasm.
Protein kinase C activity is rate limiting for shedding of the interleukin-6 receptor.
Molecular regulation of B lymphocyte response.
TLDR
Results indicated that the helper function of T cells in antibody response was mediated by soluble factors, one of the central issues in immunology.
Interleukin 6 enhancement of interleukin 3-dependent proliferation of multipotential hemopoietic progenitors.
TLDR
Results indicate that IL-3 and IL-6 act synergistically to support the proliferation of hemopoietic progenitors and that at least part of the effect results from a decrease in the G0 period of the individual stem cells.
Production of single-stranded plasmid DNA.
A catalogue of splice junction sequences.
  • S. Mount
  • Biology
    Nucleic acids research
  • 1982
TLDR
The sequence CAAG/GTAGAGT was found to be a consensus of 139 exon-intron boundaries (or donor sequences) and (TC)nNCTAG/G was found of 130 intron-exon boundaries ( or acceptor sequences).
[2] New M13 vectors for cloning
...
...