Soluble interleukin‐6 receptors released from T cell or granulocyte/macrophage cell lines and human peripheral blood mononuclear cells are generated through an alternative splicing mechanism

@article{Horiuchi1994SolubleIR,
  title={Soluble interleukin‐6 receptors released from T cell or granulocyte/macrophage cell lines and human peripheral blood mononuclear cells are generated through an alternative splicing mechanism},
  author={Sankichi Horiuchi and Yoshio Koyanagiu and Yongwei Zhouu and Hirokuni Miyamotou and Yuetsu Tanakau and Michinori Waki and Akiyoshi Matsumoto and Mikio Yamamotou and Naoki Yamamotof},
  journal={European Journal of Immunology},
  year={1994},
  volume={24}
}
To detect transcripts encoding the interleukin‐6 receptor (IL‐6R) molecule lacking the transmembrane (TM) domain, in various cell lines and peripheral blood mononuclear cells (PBMC), we used the polymerase chain reaction (PCR) with primer pairs that flank the TM domain and which were selected to generate a 398‐bp fragment. We detected 398‐bp and 304‐bp DNA molecules in the PCR products of the U1, J22HL60, MT‐2, MT‐4, U937 and HL60 cell lines and of PBMC isolated from several individuals. The… 
High‐level production of alternatively spliced soluble interleukin‐6 receptor in serum of patients with adult T‐cell leukaemia/HTLV‐I‐associated myelopathy
TLDR
It is confirmed that alternative splicing of IL‐6R mRNA is of consequence in ATL, HAM and in some autoimmune diseases, and the HTLV‐I‐infected T’cells appeared to play an important role in AS‐sIL‐ 6R production.
Elevated Serum Levels of the Soluble Form of gp130, the IL‐6 Signal Transducer, in HTLV‐1 Infection and No Involvement of Alternative Splicing for Its Generation
TLDR
Results indicate that the generation of sgp130 may not be due to an alternative splicing mechanism, as compared to normal healthy individuals and HTLV‐1‐positive cell lines, which is correlated with disease severity.
Major role of the soluble interleukin‐6/interleukin‐6 receptor complex for the proliferation of interleukin‐6‐dependent human myeloma cell lines
TLDR
The results show that the levels of circulating sIL‐ 6R (and thus those of IL‐6/sIL‐6R complex) are worth looking at in pathologies involving IL‐8 hyperactivity, and the major role of the sIL-6R for sustaining the proliferation of these cell lines.
Multilevel Regulation of IL‐6R by IL‐6–sIL‐6R Fusion Protein According to the Primitiveness of Peripheral Blood‐Derived CD133+ Cells
TLDR
It is assumed that sIL‐6R produced by shedding should be involved in autocrine and paracrine loops in the HSC microenvironment and expression and production of IL‐ 6R are tightly regulated and stage specific.
Human T‐cell leukemia virus type‐I Tax induces expression of interleukin‐6 receptor (IL‐6R): Shedding of soluble IL‐6R and activation of STAT3 signaling
Human T‐cell leukemia virus type‐I (HTLV‐I) encodes for the viral protein Tax, which is known to significantly disrupt transcriptional control of cytokines, cytokine receptors and other
Shedding of the soluble IL‐6 receptor is triggered by Ca2+ mobilization, while basal release is predominantly the product of differential mRNA splicing in THP‐1 cells
TLDR
The divergent control of these sIL‐6R isoforms indicates that they may independently influence the inflammatory response.
Soluble interleukin‐6 receptor strongly increases the production of acute‐phase protein by hepatoma cells but exerts minimal changes on human primary hepatocytes
TLDR
The results show that the expression of IL‐ 6R is low in the hepatoma cell PLC/PRF/5 when compared with primary hepatocytes and that this difference can, at least partly, explain their deficient responsiveness to IL‐6.
Production of soluble granulocyte colony-stimulating factor receptors from myelomonocytic cells.
TLDR
Two isoforms of sG-CSFR are physiologically secreted from relatively mature myeloid cells and might play an important role in myelopoiesis through their binding to serum G-CSF.
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