Soluble interleukin‐6 receptors released from T cell or granulocyte/macrophage cell lines and human peripheral blood mononuclear cells are generated through an alternative splicing mechanism

  title={Soluble interleukin‐6 receptors released from T cell or granulocyte/macrophage cell lines and human peripheral blood mononuclear cells are generated through an alternative splicing mechanism},
  author={Sankichi Horiuchi and Yoshio Koyanagiu and Yongwei Zhouu and Hirokuni Miyamotou and Yuetsu Tanakau and Michinori Waki and Akiyoshi Matsumoto and Mikio Yamamotou and Naoki Yamamotof},
  journal={European Journal of Immunology},
To detect transcripts encoding the interleukin‐6 receptor (IL‐6R) molecule lacking the transmembrane (TM) domain, in various cell lines and peripheral blood mononuclear cells (PBMC), we used the polymerase chain reaction (PCR) with primer pairs that flank the TM domain and which were selected to generate a 398‐bp fragment. We detected 398‐bp and 304‐bp DNA molecules in the PCR products of the U1, J22HL60, MT‐2, MT‐4, U937 and HL60 cell lines and of PBMC isolated from several individuals. The… 
High‐level production of alternatively spliced soluble interleukin‐6 receptor in serum of patients with adult T‐cell leukaemia/HTLV‐I‐associated myelopathy
It is confirmed that alternative splicing of IL‐6R mRNA is of consequence in ATL, HAM and in some autoimmune diseases, and the HTLV‐I‐infected T’cells appeared to play an important role in AS‐sIL‐ 6R production.
Elevated Serum Levels of the Soluble Form of gp130, the IL‐6 Signal Transducer, in HTLV‐1 Infection and No Involvement of Alternative Splicing for Its Generation
Results indicate that the generation of sgp130 may not be due to an alternative splicing mechanism, as compared to normal healthy individuals and HTLV‐1‐positive cell lines, which is correlated with disease severity.
Major role of the soluble interleukin‐6/interleukin‐6 receptor complex for the proliferation of interleukin‐6‐dependent human myeloma cell lines
The results show that the levels of circulating sIL‐ 6R (and thus those of IL‐6/sIL‐6R complex) are worth looking at in pathologies involving IL‐8 hyperactivity, and the major role of the sIL-6R for sustaining the proliferation of these cell lines.
Multilevel Regulation of IL‐6R by IL‐6–sIL‐6R Fusion Protein According to the Primitiveness of Peripheral Blood‐Derived CD133+ Cells
It is assumed that sIL‐6R produced by shedding should be involved in autocrine and paracrine loops in the HSC microenvironment and expression and production of IL‐ 6R are tightly regulated and stage specific.
Human T‐cell leukemia virus type‐I Tax induces expression of interleukin‐6 receptor (IL‐6R): Shedding of soluble IL‐6R and activation of STAT3 signaling
Human T‐cell leukemia virus type‐I (HTLV‐I) encodes for the viral protein Tax, which is known to significantly disrupt transcriptional control of cytokines, cytokine receptors and other
Shedding of the soluble IL‐6 receptor is triggered by Ca2+ mobilization, while basal release is predominantly the product of differential mRNA splicing in THP‐1 cells
The divergent control of these sIL‐6R isoforms indicates that they may independently influence the inflammatory response.
Soluble interleukin‐6 receptor strongly increases the production of acute‐phase protein by hepatoma cells but exerts minimal changes on human primary hepatocytes
The results show that the expression of IL‐ 6R is low in the hepatoma cell PLC/PRF/5 when compared with primary hepatocytes and that this difference can, at least partly, explain their deficient responsiveness to IL‐6.
Production of soluble granulocyte colony-stimulating factor receptors from myelomonocytic cells.
Two isoforms of sG-CSFR are physiologically secreted from relatively mature myeloid cells and might play an important role in myelopoiesis through their binding to serum G-CSF.


Antigens in an adult T‐cell leukemia virus‐producer cell line: Reactivity with human serum antibodies
Sera from patients with adult T‐cell leukemia (ATL) or other diseases and from healthy adults, whose titers of antibodies against ATL‐associated antigens (ATLA) had been determined by indirect
Interferon beta 2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells.
It is demonstrated that monocyte-derived hepatocyte-stimulating factor and IFN-beta 2 share immunological and functional identity and that IFN -beta 2, also known as B-cell stimulatory factor and hybridoma plasmacytoma growth factor, has the hepatocyte as a major physiologic target and thereby is essential in controlling the hepatic acute phase response.
Soluble cytokine receptors are present in normal human urine
This finding, together with the already known presence of urinary TNF binding proteins and a soluble IL-2-R both in plasma and in urine, indicates that release of soluble cytokine receptors into body fluids is a general phenomenon that occurs under normal physiological conditions.
Identification of a neutralization epitope on the envelope gp46 antigen of human T cell leukemia virus type I and induction of neutralizing antibody by peptide immunization.
Results show that the HTLV-I neutralization epitope recognized by LAT-27 locates to the gp46 amino acids 191-196, and that immunization with a peptide containing the LAT- 27 epitope can elicit an HTLV -I neutralizing antibody response.
Human B-cell differentiation factor defined by an anti-peptide antibody and its possible role in autoantibody production.
  • T. Hirano, T. Taga, F. Sakiyama
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1987
The results show that B SF-2 functions in vivo as well and suggest that the constitutive production of BSF-2 may be involved in autoantibody production, since patients with cardiac myxoma and uterine carcinoma showed autoant ibody production.
Analysis of rev gene function on human immunodeficiency virus type 1 replication in lymphoid cells by using a quantitative polymerase chain reaction method
The conventional polymerase chain reaction method is modified for general use as an extremely sensitive procedure for quantitative analysis of RNA species and shows that the ultimate effect of Rev is to direct the appearance of unspliced or singly spliced RNAs in the cytoplasm.
Molecular regulation of B lymphocyte response.
Results indicated that the helper function of T cells in antibody response was mediated by soluble factors, one of the central issues in immunology.