Soluble human interleukin-6 receptor. Expression in insect cells, purification and characterization.

@article{Weiergrber1995SolubleHI,
  title={Soluble human interleukin-6 receptor. Expression in insect cells, purification and characterization.},
  author={Oliver H. Weiergr{\"a}ber and Ulrike Hemmann and Andrea K{\"u}ster and G M{\"u}ller-newen and Jens Schneider and Stefan Rose-John and P Kurschat and Jan P. G. Brakenhoff and Michael H. Hart and Silvia Stabel},
  journal={European journal of biochemistry},
  year={1995},
  volume={234 2},
  pages={661-9}
}
The extracellular domain of the human interleukin-6 (IL-6) receptor, comprising 339 amino acids following the signal peptide, has been expressed in baculovirus-infected insect cells (Sf158). When the soluble receptor secreted into the culture medium was purified by affinity chromatography, using IL-6 immobilized on Sepharose, 6 mg soluble receptor was isolated from 1 l conditioned medium of Sf158 suspension cultures. A molar absorption coefficient of 9.3 x 10(4) l.mol-1.cm-1 was calculated from… CONTINUE READING

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