Soluble expression of a pro-transglutaminase from Streptomyces mobaraensis in Escherichia coli

@inproceedings{Marx2008SolubleEO,
  title={Soluble expression of a pro-transglutaminase from Streptomyces mobaraensis in Escherichia coli},
  author={Christian K. Marx and Thomas C. Hertel and Markus Pietzsch},
  year={2008}
}
Microbial pro-transglutaminase (MTG) from Streptomyces mobaraensis was cloned and expressed for the first time as a soluble protein at high levels in Escherichia coli. According to SDS-PAGE, more than 90% of the transglutaminase was produced in soluble form. This high solubility was obtained either by using a lactose auto-induction medium at a cultivation temperature of 28 ◦C or a temperature shift strategy comprising of growth at 37 ◦C and a post-induction temperature of 24 ◦C in a… CONTINUE READING
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