Soluble expression and characterization of a GFP-fused pea actin isoform (PEAc1)

@article{Liu2004SolubleEA,
  title={Soluble expression and characterization of a GFP-fused pea actin isoform (PEAc1)},
  author={Ai Xiao Liu and Shao Bin Zhang and X J Xu and Dong Tao Ren and Guo Qin Liu},
  journal={Cell Research},
  year={2004},
  volume={14},
  pages={407-414}
}
ABSTRACTA pea actin isoform PEAc1 with green fluorescent protein (GFP) fusion to its C-terminus and His-tag to its N-terminus, was expressed in prokaryotic cells in soluble form, and highly purified with Ni-Chelating Sepharose™ Fast Flow column. The purified fusion protein (PEAc1-GFP) efficiently inhibited DNase I activities before polymerization, and activated the myosin Mg-ATPase activities after polymerization. The PEAc1-GFP also polymerized into green fluorescent filamentous structures with… CONTINUE READING

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