Soluble expression and characterization of a GFP-fused pea actin isoform (PEAc1)


ABSTRACTA pea actin isoform PEAc1 with green fluorescent protein (GFP) fusion to its C-terminus and His-tag to its N-terminus, was expressed in prokaryotic cells in soluble form, and highly purified with Ni-Chelating Sepharose™ Fast Flow column. The purified fusion protein (PEAc1-GFP) efficiently inhibited DNase I activities before polymerization, and… (More)
DOI: 10.1038/


6 Figures and Tables