Soluble enzyme system for vitamin K-dependent carboxylation.

  title={Soluble enzyme system for vitamin K-dependent carboxylation.},
  author={Darcy Mack and Ed Suen and J M. Girardot and Judith A. Miller and Richard R Delaney and Billy C. Johnson},
  journal={The Journal of biological chemistry},
  volume={251 11},
The vitamin K-dependent carboxylating system has been solubilized by Lubrol PX or Triton X-100 treatment of vitamin K-deficient rat liver microsomes. As obtained from vitamin K-deficient rat liver, this soluble preparation is dependent upon the in vitro addition of vitamin K1 for carboxylating activity. The enzyme system is complex and is dependent upon NADH and dithiothreitol for maximum activity. While detergents used to solubilize the enzyme complex do markedly inhibit the activity of the… CONTINUE READING


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