Soluble, folded and active subtilisin in a protic ionic liquid.

Abstract

The activity of proteases chymotrypsin and subtilisin dissolved in a range of protic hydroxylalkylammonium ionic liquids was tested against the model substrate APEE (N-acetyl-L-phenylalanine ethyl ester); activity was only observed for subtilisin in diethanolammonium chloride (DEA Cl), while chymotrypsin was not active in any PIL tested. 
DOI: 10.1039/b916497a

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Cite this paper

@article{Falcioni2010SolubleFA, title={Soluble, folded and active subtilisin in a protic ionic liquid.}, author={Francesco Falcioni and Hazel R Housden and Zhenlian Ling and Seishi Shimizu and Adam J. Walker and Neil. C. Bruce}, journal={Chemical communications}, year={2010}, volume={46 5}, pages={749-51} }