Solubilized DNA-dependent RNA polymerase from human placenta: A Mn(2+)-dependent enzyme.

@article{Voigt1970SolubilizedDR,
  title={Solubilized DNA-dependent RNA polymerase from human placenta: A Mn(2+)-dependent enzyme.},
  author={H -P. Voigt and Ralph Kaufmann and Heinrich Matthaei},
  journal={FEBS letters},
  year={1970},
  volume={10 4},
  pages={257-260}
}
The 100-fold purified RNA polymerase activity from human placenta is completely dependent upon added DNA. The enzyme is most active at 3 mM Mn(2+) in the presence of 100 mM (NH(4))(2)SO(4). Denatured DNA is a better template than native DNA. alpha-Amanitin completely inhibits the incorporation of 3H-UMP, while rifampicin has no influence upon the enzymatic activity.