Solubilization of rhamnogalacturonan I galactosyltransferases from membranes of a flax cell suspension

Abstract

Galactosyltransferases (GalTs), capable of transferring a galactosyl residue from UDP-galactose (UDP-Gal) to polysaccharide acceptor, were solubilized from flax (Linum usitatissimum L.) membranes using 0.5% CHAPS. The observed requirement for a rhamnogalacturonan I (RG-I) exogenous substrate to stimulate the solubilized GalT activity provided the first… (More)
DOI: 10.1007/s004250100539

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