Solubilization of native actin monomers from human erythrocyte membranes.

@article{Tilley1986SolubilizationON,
  title={Solubilization of native actin monomers from human erythrocyte membranes.},
  author={L Tilley and M R Dwyer and Greg B. Ralston},
  journal={Australian journal of biological sciences},
  year={1986},
  volume={39 2},
  pages={117-24}
}
Up to 50% of the actin in erythrocyte membranes can be solubilized at low ionic strength in a form capable of inhibiting DNAse I, in the presence of 0.4 mM ATP and 0.05 mM calcium. In the absence of calcium and ATP, actin is released but is apparently rapidly denatured. Solubilization of G-actin increases with temperature up to 37 degrees C. At higher… CONTINUE READING