Solubilization of A1 adenosine receptor from pig brain: characterization and evidence of the role of the cell membrane on the coexistence of high- and low-affinity states.

@article{Casad1990SolubilizationOA,
  title={Solubilization of A1 adenosine receptor from pig brain: characterization and evidence of the role of the cell membrane on the coexistence of high- and low-affinity states.},
  author={Vicent Casad{\'o} and Carles Cant{\'i} and Josefa Mallol and Enric I. Canela and Carmen Lluis and Rafael Franco},
  journal={Journal of neuroscience research},
  year={1990},
  volume={26 4},
  pages={461-73}
}
The present solubilization strategy recognizes the important role of detergent cocktails in the solubilization and subsequent stability of adenosine A1, receptors from pig brain cortical membranes. The 3-[3-(cholamidopropyl)dimethylammonio]-1-propane-sulfonate-digitonin mixture produced the extraction of up to 52% of the receptor with an enrichment of 1.2-fold with respect to crude membranes. The binding activity of the soluble extract was very stable even in the absence of glycerol. In crude… CONTINUE READING

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