Solubilization and refolding of bacterial inclusion body proteins.

  title={Solubilization and refolding of bacterial inclusion body proteins.},
  author={Surinder Mohan Singh and Amulya Kumar Panda},
  journal={Journal of bioscience and bioengineering},
  volume={99 4},
Inclusion bodies produced in Escherichia coli are composed of densely packed denatured protein molecules in the form of particles. Refolding of inclusion body proteins into bioactive forms is cumbersome, results in poor recovery and accounts for the major cost in production of recombinant proteins from E. coli. With new information available on the structure and function of protein aggregates in bacterial inclusion bodies, it has been possible to develop improved solubilization and refolding… CONTINUE READING
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