Solubilization and reconstitution of an anion-sensitive H+-ATPase from corn roots


The distribution of corn root microsomal ATPase activity on continuous sucrose gradients was nearly identical to that of membrane vesicles capable of ATP-dependent H+ transport when assayed in the presence of 20 μm vanadate, 3mm azide, 5mm MgSO4 and 50mm LiCl. These assay conditions were used to monitor solubilization of an anion-sensitive H+-ATPase from a… (More)
DOI: 10.1007/BF01870796

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