Solubilization and purification of the alpha 1-adrenergic receptor using a novel affinity resin.

Abstract

The highly selective alpha 1-adrenergic receptor antagonist prazosin was used to identify binding sites having alpha-adrenergic specificity in rat hepatic plasma membranes. Solubilization of the membrane-bound receptors was achieved by incubation with the nonionic detergent digitonin, and binding activity was assayed by using [3H]prazosin and a polyethylene… (More)

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@article{Graham1982SolubilizationAP, title={Solubilization and purification of the alpha 1-adrenergic receptor using a novel affinity resin.}, author={Robert M. Graham and Hayato Hess and Charles J. Homcy}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={1982}, volume={79 7}, pages={2186-90} }