Solubilization and partial characterization of rat liver squalene epoxidase.

  title={Solubilization and partial characterization of rat liver squalene epoxidase.},
  author={T. Ono and K. Bloch},
  journal={The Journal of biological chemistry},
  volume={250 4},
  • T. Ono, K. Bloch
  • Published 1975
  • Chemistry, Medicine
  • The Journal of biological chemistry
  • The microsomal enzyme system from rat liver which catalyzes squalene epoxidation requires a supernatant protein and phospholipids (Tai, H., and Bloch, K. (1972) J. Biol. Chem. 247, 3767). It has now been found that these two cytoplasmic components can be replaced by Triton X-100. The same detergent solubilizes the microsomal squalene epoxidase and the resulting supernatant can be separated into two components, A and B, by DEAE-cellulose chromatography. Neither Fraction A nor B alone has… CONTINUE READING
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