Solubilization and denaturation of monomeric actin from erythrocyte membranes by p-mercuribenzenesulfonate.

@article{Gordon1990SolubilizationAD,
  title={Solubilization and denaturation of monomeric actin from erythrocyte membranes by p-mercuribenzenesulfonate.},
  author={Siamon Gordon and Greg B. Ralston},
  journal={Biochimica et biophysica acta},
  year={1990},
  volume={1025 1},
  pages={43-8}
}
Solutions of p-mercuribenzenesulfonate extract the peripheral proteins from the red cell membrane in a water-soluble form. Low concentrations of the reagent selectively solubilize actin, while at higher concentrations, spectrin, ankyrin and bands 4.2 and 4.1 are extracted. After brief exposure to the reagent, followed by displacement of the mercurial with dithiothreitol or 2-mercaptoethanol, the soluble actin is capable of inhibiting DNAse I activity. With prolonged exposure or with higher… CONTINUE READING