Solubilization, two-dimensional separation and detection of the cardiac myofilament protein troponin T.

Abstract

Proteomics strongly relies on the separation of complex protein mixtures, with two-dimensional electrophoresis (2-DE) being a commonly used technique. However efficient separation requires adequate solubilization of the original sample which will determine whether all proteins are accurately represented. Cardiac muscle has presented a particular challenge… (More)

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