Solubilization, partial purification, and reconstitution of glutamate- and N-methyl-D-aspartate-activated cation channels from brain synaptic membranes.

@article{Ly1991SolubilizationPP,
  title={Solubilization, partial purification, and reconstitution of glutamate- and N-methyl-D-aspartate-activated cation channels from brain synaptic membranes.},
  author={A M Ly and Elias K. Michaelis},
  journal={Biochemistry},
  year={1991},
  volume={30 17},
  pages={4307-16}
}
L-Glutamate-activated cation channel proteins from rat brain synaptic membranes were solubilized, partially purified, and reconstituted into liposomes. Optimal conditions for solubilization and reconstitution included treatment of the membranes with nonionic detergents in the presence of neutral phospholipids plus glycerol. The affinity batch chromatography… CONTINUE READING