Solid-state extraction of cortisol from plasma or serum for liquid chromatography.

Abstract

Solid-state extraction of plasma or serum offers significant advantages over traditional liquid-liquid methods in cortisol determinations by liquid chromatography (HPLC). Sample preparation is facilitated and HPLC column contamination is minimized. Extract yield from the solid-state columns is enhanced by sample pretreatment with methanol. Cortisol values by solid-state extraction/HPLC correlate well (r = 0.92) with those from a commercial radioimmunoassay procedure.

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Cite this paper

@article{Hofreiter1983SolidstateEO, title={Solid-state extraction of cortisol from plasma or serum for liquid chromatography.}, author={Birgit Hofreiter and Ales Mizera and John P . Allen and Anthony M Masi and W C Hicok}, journal={Clinical chemistry}, year={1983}, volume={29 10}, pages={1808-9} }