Solid phase radioimmunoassay of apolipoprotein B (apo B) in normal human plasma.

@article{Thompson1976SolidPR,
  title={Solid phase radioimmunoassay of apolipoprotein B (apo B) in normal human plasma.},
  author={Gilbert R. Thompson and Maria E. Birnbaumer and Robert I. Levy and Antonio M. Gotto},
  journal={Atherosclerosis},
  year={1976},
  volume={24 1-2},
  pages={107-18}
}
A solid phase radioimmunoassay (RIA) has been developed for apolipoprotein B (apoB), a major constituent of very low density lipoprotein (VLDL) and low density lipoprotein (LDL) in man. Antisera were prepared against apoB in goats and rabbits, coupled to bromoacetyle cellulose, and the complex was incubated with [125I] LDL. The RIA was based on the displacement of [ 1252]-LDL by unknown samples, as compared with unlabeled LDL standards, using a logit transformation to calculate results. The RIA… CONTINUE READING