Solid-phase permethylation for glycomic analysis.

  title={Solid-phase permethylation for glycomic analysis.},
  author={Yehia Mechref and Pilsoo Kang and Milos V. Novotny},
  journal={Methods in molecular biology},
This chapter discusses in detail a miniaturized version of the widely used permethylation technique which permits quantitative derivatization of oligosaccharides derived from minute quantities of glycoprotein. The approach involves packing of sodium hydroxide powder or beads in a microcolumn format, including spin columns, fused silica capillaries (500… CONTINUE READING