Solid-phase microextraction as a sample preparation strategy for the analysis of seleno amino acids by gas chromatography-inductively coupled plasma mass spectrometry.

@article{Vonderheide2002SolidphaseMA,
  title={Solid-phase microextraction as a sample preparation strategy for the analysis of seleno amino acids by gas chromatography-inductively coupled plasma mass spectrometry.},
  author={Anne P. Vonderheide and M Montes-Bay{\'o}n and Joseph A Caruso},
  journal={The Analyst},
  year={2002},
  volume={127 1},
  pages={49-53}
}
Solid-phase microextraction (SPME) is used as a sample preparation strategy for gas chromatographic (GC) analysis of the seleno amino acids, selenomethionine (SeMet), selenoethionine (SeEt) and selenocystine (SeCys). Acylation of the amino group and esterification of the carboxylic group in these compounds was performed with isobutylchloroformate to increase volatility. The amino acid derivatives were then extracted by silica fibers with polydimethylsiloxane (PDMS) coatings prepared by the sol… CONTINUE READING