Solid-phase extraction of N-linked glycopeptides

@article{Tian2007SolidphaseEO,
  title={Solid-phase extraction of N-linked glycopeptides},
  author={Yuan Tian and Yong Zhou and Sarah Eleanor Elliott and Ruedi Aebersold and Hui Zhang},
  journal={Nature Protocols},
  year={2007},
  volume={2},
  pages={334-339}
}
Protein glycosylation is a common post-translational modification and has been increasingly recognized as one of the most prominent biochemical alterations associated with malignant transformation and tumorigenesis. N-linked glycosylation is prevalent in proteins on the extracellular membrane, and many clinical biomarkers and therapeutic targets are glycoproteins. Here, we describe a protocol for solid-phase extraction of N-linked glycopeptides and subsequent identification of N-linked… CONTINUE READING
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