Solid phase extraction and liquid chromatographic determination of sildenafil and N-demethylsildenafil in rat serum with basic mobile phase.

@article{Guermouche2006SolidPE,
  title={Solid phase extraction and liquid chromatographic determination of sildenafil and N-demethylsildenafil in rat serum with basic mobile phase.},
  author={M Hassan Guermouche and Karim Bensalah},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2006},
  volume={40 4},
  pages={952-7}
}
HPLC method for the determination of sildenafil and its metabolite (N-demethylsildenafil) in rat serum has been developed. The technique included a solid phase extraction of the serum samples on a [poly(divinylbenzene-co-N-vinylpyrrolidone)] solid phase extraction sorbent. After conditioning, the cartridge was loaded with 0.5 mL of buffered serum containing internal standard. Elution was made with 1 mL of acetonitrile. After evaporation of the eluates to dryness and reconstitution with methanol… CONTINUE READING